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Five Prime Presents Preclinical Research Data on CSF-1R and PD-1 Blockade at the 2017 AACR Annual Meeting

On April 3, 2017 Five Prime Therapeutics, Inc. (Nasdaq:FPRX), a clinical-stage biotechnology company focused on discovering and developing innovative immuno-oncology protein therapeutics, reported that a poster featuring preclinical data related to Five Prime’s CSF-1R antibody, cabiralizumab (FPA008), was presented today at the 2017 American Association for Cancer Research (AACR) (Free AACR Whitepaper) Annual Meeting in Washington, D.C (Press release, Five Prime Therapeutics, APR 3, 2017, View Source [SID1234518423]). The poster titled "Antibody-Based Inhibition of CSF-1R as a Component of Combination Immunotherapy in Preclinical Models" is available at View Source

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"These preclinical findings suggest that the surrogate antibody for FPA008 significantly reduces tumor growth in murine syngeneic tumor models when administered alone and when administered in combination with a PD-1 antibody," said Kevin Baker, Ph.D., Senior Vice President of Development Sciences at Five Prime. "These findings support previous preclinical data that served as the basis for our Phase 1a/1b clinical trial that we are running in collaboration with Bristol-Myers Squibb (BMS) to investigate the use of cabiralizumab in combination with nivolumab (anti-PD-1, OPDIVO) in six different tumor types."

The colony stimulating factor 1 receptor (CSF-1R) signaling pathway promotes tumor progression via the recruitment, differentiation, and survival of immuno-suppressive, M2 polarized, tumor-associated macrophages (TAMs). Five Prime has developed cabiralizumab (FPA008), an IgG4 antibody against CSF-1R that blocks the ability of both CSF-1 and IL-34 to bind and activate this receptor, thereby modulating the immune response to tumorigenesis.

Five Prime has identified alterations in the tumor microenvironment that occur upon CSF-1R inhibition, including significant reduction of immunosuppressive M2 TAMs and an increase in tumor PD-L1 expression. Notably, when dosing in combination, the blockade of CSF-1R and PD-1/PD-L1 results in a significant increase in CD8+ T cells within the tumor. The results show that, when added to PD-1/PD-L1 blockade, the surrogate antibody for FPA008 can significantly enhance anti-tumor efficacy.

Five Prime Presents Data on Unique Immuno-Oncology Discovery Research at the 2017 AACR Annual Meeting

On April 3, 2017 Five Prime Therapeutics, Inc. (Nasdaq:FPRX), a clinical-stage biotechnology company focused on discovering and developing innovative immuno-oncology protein therapeutics, reported that a poster featuring data related to Five Prime’s unique discovery platform was presented today at the 2017 American Association for Cancer Research (AACR) (Free AACR Whitepaper) Annual Meeting in Washington, D.C (Press release, Five Prime Therapeutics, APR 3, 2017, View Source [SID1234518422]). The poster titled "Identification of Novel T Cell Co-Inhibitory and Co-Stimulatory Receptors from Screening a Comprehensive Library of Extracellular Proteins" is available at View Source

"We have generated a comprehensive library of essentially all human extracellular proteins and have used this library to screen for new agents that modulate the function of various immune cell subsets," said Luis Borges, Ph.D., Senior Vice President of Research at Five Prime. "Taken together, we have developed robust in vitro and in vivo platforms that allow us to discover new immuno-oncology targets that we believe will help address the needs of cancer patients who fail to respond to current immunomodulatory therapies."

Five Prime has developed multiple sophisticated in vitro, in vivo, and biophysical assay systems for screening its comprehensive library of human extracellular proteins to identify novel therapeutics that can reprogram immune cells in the tumor microenvironment.

These multiple approaches include:

In vitro screens of primary human effector and regulatory T cells to identify novel proteins that can modulate T cell activation and suppression.
Screening subsets of the discovery library in four distinct syngeneic mouse tumor models (either as single agents, or in combination with checkpoint blockade or a vaccine) to identify targets that can modulate an anti-tumor immune response in vivo.
Performing a combinatorial biophysical screen for extracellular protein-protein interactions within a set of approximately 700 predicted immune-related transmembrane proteins to identify novel receptor-ligand interaction networks.
As a result of these screens that combine data from multiple screening approaches, Five Prime has prioritized a series of new targets for validation and potential generation of novel immuno-oncology therapeutics.

Sierra Oncology Collaborator ICR Reports Preclinical Synthetic Lethality Data for Chk1 Inhibitor SRA737 at AACR

On April 3, 2017 Sierra Oncology, Inc. (NASDAQ: SRRA), a clinical stage drug development company focused on advancing next generation DNA Damage Response therapeutics for the treatment of patients with cancer, reported that preclinical results for its Chk1 inhibitor, SRA737, were presented on April 2 in a poster at the American Association of Cancer Research (AACR) (Free AACR Whitepaper) Annual Meeting being held in Washington D.C (Press release, Sierra Oncology, APR 3, 2017, View Source [SID1234518421]). This research was conducted in the laboratory of Professor Paul Workman, Chief Executive and President of The Institute of Cancer Research (ICR), London, UK, and funded by Wellcome.

"These data demonstrate that inhibition of B-family DNA polymerases, POLA1, POLE and POLE2, combined with inhibition of Chk1, is synthetically lethal in numerous cancer cell lines," noted Professor Workman. "This supports the genetically-driven clinical development strategy Sierra intends to pursue for SRA737. For example, subsets of colon and endometrial cancers with POLE mutations may be sensitive to CHK1 inhibitors as monotherapy. In addition, gemcitabine is known to impair the B-family DNA polymerases, further supporting the rationale for its clinical combination with SRA737."

"The ICR is a world-class cancer research institution and we are fortunate to maintain an ongoing collaborative relationship for the development of SRA737. In particular, the data reported by Professor Workman’s laboratory continues to reinforce the synthetic lethality hypothesis for SRA737, and further validates our approach to developing our lead drug," said Dr. Nick Glover, President and CEO of Sierra Oncology. "Appropriate patient selection is critical for clinical success. Fortunately, the biology underlying Chk1’s role in cancer and the DNA Damage Response suggest numerous opportunities where a patient’s specific tumor genetics might be linked to their predicted response to SRA737."

The poster, entitled: "Screening the druggable genome for synthetic lethal interactions with the CHK1 inhibitor PNT737" is available at www.sierraoncology.com. Additional information including full abstracts can be found at www.aacr.org.

About SRA737
SRA737, is a highly selective, orally bioavailable small molecule inhibitor of Checkpoint kinase 1 (Chk1), currently being evaluated in two Phase 1 clinical trials in patients with advanced cancer.

The first trial is evaluating SRA737’s potential to induce synthetic lethality as monotherapy. Sierra recently proposed amendments to this trial to prospectively enroll subjects with tumors identified to have genetic aberrations hypothesized to confer sensitivity to Chk1 inhibition. The study will employ a genetic selection algorithm targeting mutations in TP53, BRCA1, BRCA2, MYC, RAS, or other related genes associated with tumor suppression, oncogenesis, DNA damage repair, and replicative stress. Additionally, Sierra intends to modify the study to assess SRA737’s clinical activity across several cancer indications that harbor these genetic mutations including, for example, cohorts of subjects with 1) previously treated metastatic colorectal cancer; 2) platinum-resistant ovarian cancer; 3) metastatic castration-resistant prostate cancer; 4) advanced non-small cell lung cancer; and 5) advanced head and neck squamous cell carcinoma.

Sierra is currently also evaluating SRA737 in combination with gemcitabine and gemcitabine plus cisplatin. The company intends to modify the design of this clinical trial to expand enrollment in the gemcitabine combination following the completion of dose escalation. Subjects with 1) advanced bladder cancer and 2) advanced pancreatic cancer will be enrolled into genetically-selected dose expansion cohorts, employing a genetic selection algorithm similar to that to be used in the monotherapy protocol.

Digital Spatial Profiling Technology Enables Quantitative Protein Profiling in FFPE Tissue Providing Detailed Molecular Characterization of Immune Response to Tumors

On April 3, 2017 NanoString Technologies, Inc. (NASDAQ:NSTG), a provider of life science tools for translational research and molecular diagnostic products, reported the validation of its novel Digital Spatial Profiling (DSP) technology by the current state-of-the-art AQUA method for quantitating protein targets in FFPE (Press release, NanoString Technologies, APR 3, 2017, View Source [SID1234518420]). The data was generated through a collaboration between NanoString and Dr. David Rimm’s lab at the Yale School of Medicine as part of a Technology Access Program for NanoString’s DSP technology. The data will be presented in a poster session at the American Association for Cancer Research (AACR) (Free AACR Whitepaper) conference, being held April 1-5, 2017 in Washington, D.C.

"We are excited to be working with NanoString on their new Digital Spatial Profiling technology. Our AQUA quantitative fluorescence approach has been limited to 4-5 fold multiplexing, where DSP can currently multiplex up to 50 protein analytes and has the potential to measure hundreds of RNAs simultaneously on the same tissue section," said Dr. David L. Rimm, Professor of Pathology and of Medicine (Medical Oncology); Director of Pathology Tissue Services; Director of Translational Pathology, Yale University. "This brings many advantages, including the ability to include ‘housekeeping’ proteins for standardization of assays and normalization of pre-analytic variables. Because of the variable sized sample region, it is a perfect match for the ‘field of view’ assessment we do with the AQUA technology in our lab. We designed AQUA to measure at the molecular level rather than the cellular level, and the same is true of NanoString’s DSP."

AQUA technology, which was developed by Dr. Rimm, has been demonstrated to be both reproducible and quantitative when applied to tissue-based immunoassays. This study demonstrated that both DSP and AQUA are quantitative methods for profiling protein expression, eliminating the subjectivity and variability associated with conventional immunohistochemistry techniques. However, benchmarking against AQUA revealed several key advantages of DSP technology, including the high multiplex capability and wide dynamic range. These capabilities are of particular significance when profiling tumor cell specific protein expression from the complex tumor microenvironment.

"Localized expression of immuno-regulatory proteins shapes the immune response against tumors, and the detection of cell specific proteins can reveal the presence of important immune cell populations," stated Alessandra Cesano, M.D., Ph.D, chief medical officer at NanoString. "Spatial profiling of these key molecules could lead to discovery of novel therapeutic targets and signatures that characterize immunological responses. This could enable predictive biomarkers to guide the development of immunotherapies and companion diagnostics."

Digital Spatial Profiling will be featured in two additional posters and a spotlight presentation during the AACR (Free AACR Whitepaper) meeting. Merritt et al., will present recent technical advances of the platform including single cell selection and RNA profiling. Balko et al., applied the technology to characterizing checkpoint inhibitor therapy-induced autoimmune toxicities. The spotlight presentation will feature the inventors of the technology, Dr. Gordon Mills and Dr. Joseph Beechem who will speak about the potential of the platform to enable deep molecular characterization of complex biological phenomenon and provide greater insight into tumor immune cell interactions.

NanoString is accelerating the development of the Digital Spatial Profiling technology with customers via its Technology Access Program. Under the program, customers submit up to 20 FFPE tissue sections and NanoString performs a high-plex protein spatial profiling assay with a panel of 30 pre-validated immuno-oncology targets. An assay report along with raw digital data and processed results are provided back to customers. Researchers interested in participating in NanoString’s technology access program for its Digital Spatial Profiling technology should contact the company at [email protected].

Key DSP Events at AACR (Free AACR Whitepaper) 2017

Spotlight Presentation: Monday, April 3: 10:00am – 11:00am ET Spotlight Theater B – Hall A
Powering Precision Oncology Research with 3D Biology Technology: High Plex Multi-Analyte Profiling on FFPE with Spatial Resolution.
Gordon B. Mills M.D. Ph.D., Professor and Chair of Systems Biology at MD Anderson Cancer Center and Joseph M. Beechem, PhD, Senior Vice President of Research & Development, NanoString Technologies.

Poster # 3810 – Tuesday, April 4: 8:00am – 12:00pm ET
Validation of novel high-plex protein spatial profiling quantitation based on NanoString’s Digital Spatial Profiling (DSP) technology with quantitative fluorescence (QIF) [Section #32, Board #6]
Maria I. Toki, M.D., Ph.D., Yale School of Medicine

Poster # 588 – Sunday, April 2: 1:00 PM-5:00 PM
Advanced molecular characterization of severe autoimmune toxicities associated with checkpoint inhibitor therapies [Section #25, Board #22]
Justin M. Balko, Pharm.D., Ph.D., Assistant Professor of Cancer Biology, Vanderbilt University

Poster# 3955 – Tuesday, April 4: 8:00 AM-12:00 PM
Spatially resolved, multiplexed digital characterization of protein and mRNA distribution and abundance in formalin-fixed, paraffin-embedded (FFPE) tissue sections based on NanoString’s Digital Spatial Profiling [Section #41, Board #22]
Chris Merritt, Ph.D., NanoString Technologies

Intrexon, ZIOPHARM and Merck KGaA, Darmstadt, Germany Advancing Next-Generation Non-Viral CAR-T Platform Empowered by Membrane-Bound IL-15 Under RheoSwitch Therapeutic System Control

On April 3, 2017 Intrexon Corporation (NYSE: XON), a leader in the engineering and industrialization of biology to improve the quality of life and health of the planet, ZIOPHARM Oncology (NASDAQ: ZIOP), a biopharmaceutical company focused on new immunotherapies, and Merck KGaA, Darmstadt, Germany, a leading science and technology company, reported an update on the development of next-generation chimeric antigen receptor T cell (CAR-T) therapy for cancer as part of their strategic collaboration and license agreement (Press release, Intrexon, APR 3, 2017, View Source [SID1234518419]).

Intrexon and ZIOPHARM Oncology, in an exclusive partnership with Merck KGaA, Darmstadt, Germany, are advancing a unique approach to develop therapeutic candidates for two CAR-T targets expressed on a wide range of tumor types, including hematologic malignancies and solid tumors.

The distinctive methodology centers on two technologies: the proprietary RheoSwitch Therapeutic System (RTS) platform to regulate expression of membrane-bound interleukin-15 (mbIL15) co-expressed with CARs and Sleeping Beauty non-viral gene integration.

"Sleeping Beauty and the RTS approach are a powerful combination to improve the manufacturing process and instill control over CAR-modified T cells co-expressing cytokines such as membrane-bound IL-15. The collaboration with Intrexon and Merck KGaA, Darmstadt, Germany, has been a catalyst to progress these next-generation gene therapy technologies. We are excited by the progress and look forward to advancing this innovative approach toward the clinic in mid-2018," said Laurence Cooper, M.D., Ph.D., Chief Executive Officer of ZIOPHARM.

The interleukin-15 (IL-15) cytokine is increasingly recognized as a key driver of therapeutic effect in CAR-T therapy, including in a recent Journal of Clinical Oncology publication which correlated lymphoma remissions with elevated IL-15 levels. Through the RTS gene switch, the expression of mbIL15 can be regulated to help CARs target cancers in a controlled manner, thus providing a new paradigm in T-cell therapy.

Additionally, the non-viral Sleeping Beauty transposon-transposase is an exceptional system for introducing genes encoding CARs and TCRs into T cells that holds multiple advantages over viral-based delivery systems. It simplifies genetic modification, and when coupled with reduced ex vivo processing, offers a pathway to shortened manufacturing and personalized T-cell therapies.