On November 13, 2024 Medigene AG (Medigene or the "Company", FSE: MDG1, Prime Standard), an oncology platform company focused on the research and development of T cell receptor (TCR)-guided therapies for the treatment of cancer, reported data on the newest addition to its End-to-End (E2E) Platform, an Interferon-Gamma (IFN-γ) Biosensor technology that allows real-time monitoring and quantification of IFN-γ release from cytokine-secreting cells at the Society of Immunotherapy of Cancer (SITC) (Free SITC Whitepaper) 2024 Conference taking place in Houston, TX, USA from November 6-10, 2024 (Press release, MediGene, NOV 13, 2024, View Source [SID1234648303]).
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The poster "The IFN-γ Biosensor – A universal tool for IFN-γ detection in cellular co-culture assays" is available on Medigene’s website: View Source
"Release of IFN-γ is one of the most common parameters used to measure T cell or NK cell functionality. Current methods for detection of IFN-γ secretion have several drawbacks, including extensive hands-on-time and needs for expensive, temperature-sensitive reagents," said Dolores Schendel, CSO of Medigene AG. "The IFN-γ Biosensor is an innovative tool within our E2E Platform that provides a simple, low-cost, cell-based alternative for quantification of secreted IFN-γ. This biosensor addresses limitations of current methods by reducing time, costs and complexity. The biosensor cells can be added directly as third-party bystander cells to co-cultures of T or NK cells, thereby dramatically reducing assay steps while enabling a dynamic assessment of IFN-γ secretion over time, going beyond standard end-point assays." The presented poster displayed a direct comparison between the conventional standard method Enzyme-linked Immunosorbent Assay (ELISA) and the Company´s proprietary innovative IFN-γ Biosensor to determine IFN-γ release directly in cell co-cultures or in cell supernatants. The biosensor cells were generated to produce a green fluorescent protein (GFP) when exposed to IFN-γ and allow for quantitative analysis of the GFP fluorescent reporter signal
The IFN-γ biosensor demonstrated comparable sensitivity to ELISA, effectively detecting low levels of IFN-γ using standard measurement tools like flow cytometry and fluorescence readings. Detection was highly specific for IFN-γ, with minimal response observed only when exposed to exceedingly high levels of IFN-α or IFN-β, two other interferon types. Further in vitro assays showed that the biosensor produced dose-dependent results comparable to ELISA, that were stable and sensitive to IFN-γ stimulation over extended co-culture periods. The addition of this process optimization technology to the Medigene End-to-End Platform significantly reduces costs and complexity while increasing speed and reproducibility when applied in high-throughput screening assays of T or NK cell functionality. The time from assay completion to analysis of results was significantly reduced, providing faster data processing and is more efficient and streamlined when implemented in robotic workflows.
Overall, with its broad detection range, dynamic measurements over time, and high specificity, the IFN-γ Biosensor technology to provide a sensitive and efficient alternative that extends beyond a traditional ELISA for measuring T cell and NK cell functionality. Simple readouts using either flow cytometry and fluorescence readers-supports its easy integration in high-throughput workflows, cutting costs and handling times.