CAR T-Cell Study Enabled by 10x Genomics Single Cell Technology Published in Nature Medicine

On July 5, 2023 10x Genomics, Inc. (Nasdaq: TXG), a leader in single cell and spatial biology, reported that its single-cell genomic technologies were used in a study published in the journal Nature Medicine about new gene signatures that explain why some children with leukemia have longer remission after CAR T-cell therapy (Press release, 10x Genomics, JUL 5, 2023, View Source [SID1234633064]). The collaborative research project was conducted by researchers from Great Ormond Street Hospital (GOSH), the Wellcome Sanger Institute and the UCL Great Ormond Street Institute of Child Health (UCL GOS ICH).

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Researchers conducting the study, "Transcriptional signatures associated with persisting CD19 CAR-T cells in children with leukemia," used Chromium Single Cell Gene Expression technologies and T-cell receptor sequencing of samples to look at what makes the long-lasting CAR T-cells unique. These helped identify a genetic signature of the CAR T-cells that persisted in the body long-term – an important predictor of durable remission. The data was from the CARPALL trial, which used molecular features and clonal dynamics of CD19 CAR T-cells of 10 children with relapsed or refractory B-cell acute lymphoblastic.

Ben Hindson, Co-founder and Chief Scientific Officer at 10x Genomics, said, "Single cell genomics is a powerful tool that is transforming cancer research and our understanding of health and disease. We are so proud to have 10x technologies behind this incredible breakthrough. We celebrate the achievements of the research team and the selfless dedication of the families who made this study possible. We look forward to continuing to give researchers the technologies needed to fuel new biological discoveries and ultimately help improve patient care."

Using Chromium Single Cell Gene Expression products, researchers identified a unique double negative phenotype of long-lasting CAR T-cells, which provides insights into how these cells survive and remain active in the body. These key genes appeared to enable the CAR T-cells to persist and therefore allow the children to live cancer free long-term. The findings may lead to discovering new biomarkers that could predict the long-term durability of CAR T-cell therapies and provide better treatment options for patients.

Dr. Sam Behjati, co-senior author, Group Lead and Wellcome Senior Research Fellow at the Wellcome Sanger Institute and Honorary Consultant Paediatric Oncologist at Addenbrooke’s Hospital, Cambridge, said, "This study is a fantastic step forward in our understanding of CAR T-cell persistence and illustrates the power of collaborative science and combining pioneering clinical research with cutting-edge genomic science. It is crucial that we continue to develop and build on these new treatments to help more children with leukaemia across the world."

Registrational Pivotal Phase III Study of Olverembatinib for the First-Line Treatment of Patients with Ph+ ALL Approved by the CDE in China

On July 5, 2023 Ascentage Pharma (6855.HK), a global biopharmaceutical company engaged in developing novel therapies for cancer, chronic hepatitis B (CHB), and age-related diseases, reported that the Center for Drug Evaluation (CDE) of the China National Medical Products Administration (NMPA) has approved a registrational pivotal Phase III study of olverembatinib, Ascentage Pharma’s lead drug candidate, in combination with chemotherapy (investigational arm) comparing imatinib in combination with chemotherapy (control arm) for treatment of patients with naïve Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) (Press release, Ascentage Pharma, JUL 5, 2023, View Source [SID1234633063]). This approval marks a major milestone that could potentially pave the way for olverembatinib to become the first China-approved tyrosine kinase inhibitor (TKI) for the treatment of patients with Ph+ ALL in the first-line setting.

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Accounting for 20%-30% of all ALL cases in adults, Ph+ ALL is commonly associated with a high relapse rate, short progression-free survival (PFS), and poor prognosis. Prior to the introduction of TKIs, a class of targeted small molecule compounds, allogeneic hematopoietic stem cell transplantation (allo-HSCT) after achieving complete responses (CRs) from chemotherapy was widely adopted as a first-line treatment for patients with Ph+ ALL. However, the five-year overall survival (OS) was only less than 30% and more than 70% patients relapsed before the transplantation or simply lacked access to the surgical treatment[1]. The clinical adoption of TKIs has resulted in a new clinical paradigm for patients with Ph+ ALL, although first and second-generation TKIs have considerable limitations, including high relapse rates and disappointing long-term survival with a three to five-year OS rate of just about 50%[2]. These limitations are primarily caused by low complete molecular responses (CMRs) and T315I kinase domain mutations, thus leaving substantial room for improvement in the treatment of Ph+ ALL. Currently, no TKI has been approved for the first-line treatment of Ph+ ALL in China and third-generation TKIs with more potent efficacy can potentially provide better prognosis for patients with Ph+ ALL by inducing a higher rate of CMRs and inhibiting the T315I mutation.

Ascentage Pharma’s novel drug candidate, olverembatinib, is an orally-administered third-generation TKI and the first and only China-approved third-generation BCR-ABL inhibitor. Currently, olverembatinib is being jointly commercialized by Ascentage Pharma and Innovent Biologics. Olverembatinib was approved by the CDE of the China NMPA in November 2021 for the treatment of adult patients with TKI-resistant chronic-phase chronic myeloid leukemia (CML-CP) or accelerated-phase CML (CML-AP) harboring the T315I mutation. Previously, olverembatinib received a recommendation from the Chinese Society of Clinical Oncology (CSCO) Guidelines for the Diagnosis and Treatment Hematologic Malignancies as a treatment option for patients with Ph+ ALL.

"This approval for the pivotal Phase III study in newly diagnosed patients with Ph+ ALL marks a major milestone for the development of olverembatinib," said Dr. Yifan Zhai, Chief Medical Officer of Ascentage Pharma. "TKIs combined with chemotherapy is a widely recognized standard treatment strategy for patients with Ph+ ALL, but no TKI has been approved for the first-line setting in this therapeutic area, thus potentially making olverembatinib the first TKI approved for the first-line treatment of Ph+ ALL in China. Fulfilling our mission of addressing unmet clinical needs in China and around the world, we will expeditiously advance the clinical development of olverembatinib in efforts to allow more patients to benefit from this novel therapeutic as soon as possible."

References

1. Fielding A K, Rowe JM, Richards SM, et a1. Prospective outcome data on 267 unselected adult patients with Philadelphia chromosome positive acute lymphoblastic leukemia confirms superiority of allogeneic transplantation over chemo therapy in the pre imatinib era results from the International ALL Trim MRCUKALIXII/ECOG 2993. Blood, 2009,113: 4489- 4496.
2. Elias Jabbour et. Treatment of Adults With Philadelphia Chromosome Positive Acute Lymphoblastic Leukemia-From Intensive Chemotherapy Combinations to Chemotherapy-Free Regimens: A Review. JAMA Oncol. 2022 Sep 1;8(9):1340-1348.

About Olverembatinib (HQP1351)

Developed by Ascentage Pharma with support from the National Major New Drug Discovery and Manufacturing Program in China, the orally active, third-generation TKI olverembatinib is the first and only China-approved third-generation BCR-ABL inhibitor targeting drug-resistant chronic myeloid leukemia (CML). Olverembatinib can effectively target a spectrum of BCR-ABL mutants, including the T315I mutation.

In November 2021, olverembatinib was granted a conditional approval through the Priority Review process by the China National Medical Products Administration (NMPA) for the treatment of adult patients with tyrosine kinase inhibitor (TKI)-resistant chronic-phase CML (CML-CP) or accelerated-phase CML (CML-AP) harboring the T315I mutation as confirmed by a validated diagnostic test. Subsequently, Olverembatinib was included into the China 2022 National Reimbursement Drug List (NRDL) for the approved indication. In March 2021, olverembatinib was granted a Breakthrough Therapy Designation (BTD) by the CDE for the treatment of patients with CML-CP who are resistant and/or intolerant of first- and second-generation TKIs. In July 2022, an NDA for this indication was accepted by the NMPA and subsequently granted a BTD that will support a full approval of olverembatinib.

In overseas, olverembatinib was cleared by the US FDA in July 2019 to directly enter a Phase Ib study. Since 2018, the clinical results of olverembatinib have been selected for oral presentations at the American Society of Hematology (ASH) (Free ASH Whitepaper) Annual Meetings for five consecutive years, and was nominated for "Best of ASH (Free ASH Whitepaper)" in 2019. To date, olverembatinib has been granted one Fast Track Designation (FTD) and four Orphan Drug Designations (ODDs) from the US Food and Drug Administration (FDA) for the treatment of CML, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), and gastrointestinal stromal tumour (GIST); and one Orphan Designation from the European Medicines Agency (EMA) of the European Union for the treatment of CML.

In July 2021, Ascentage Pharma (6855.HK) and Innovent Biologics (1801.HK) reached the agreement regarding the joint development and commercialization of olverembatinib in China.

*Olverembatinib is an investigational drug that has not been approved for any indication outside the Chinese mainland.

Initial Data from Allarity’s Phase 2 Trial of IXEMPRA® Indicate Potential for Improved Clinical Benefit in DRP®-Selected Metastatic Breast Cancer Patients

On July 5, 2023 Allarity Therapeutics, Inc. ("Allarity" or the "Company") (Nasdaq: ALLR), a clinical-stage pharmaceutical company developing novel oncology therapeutics together with drug-specific DRP companion diagnostics for personalized cancer care, reported initial results from its European Phase 2 clinical trial evaluating the efficacy of IXEMPRA in metastatic breast cancer (mBC) patients selected with the DRP-IXEMPRA companion diagnostic (CDx) candidate (Press release, Allarity Therapeutics, JUL 5, 2023, View Source [SID1234633061]). Researchers prescreened mBC patients using Allarity’s DRP-IXEMPRA CDx, a complex transcriptomic signature comprising multiple mRNA biomarkers of drug response/resistance. Patients were assigned a DRP-score, and those with scores above 67% were selected for treatment with IXEMPRA.

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Of the 36 patients screened with the DRP-IXEMPRA CDx, investigators identified five DRP positive patients. Among the evaluable patients assessed up to the data evaluation cut-off, there were promising signs of clinical benefit in four out of four evaluable cases:

One partial responder (PR) (tumor shrinkage of 66%).
One partial responder (PR) (tumor shrinkage of 59%).
One patient experienced 24 weeks of stable disease.
One patient experienced 19 weeks of stable disease.

" We are enthusiastic about these promising very early trial results since the observed clinical benefit rate exceed s what has been historically observed for IXEMPRA treatment without the DRP – IXEMPRA CDx patient selection. While still early, these data suggest that the use of the DRP -IXEMPRA CDx for patient selection and treatment may help identify mBC patients most able to benefit from this course of treatment . Accordingly , the DRP -IXEMPRA CDx , if approved, may provid e clinicians with an important diagnostic to guide patient treatment in this hard-to-treat population, " said Marie Foegh, M.D., Chief Medical Officer of Allarity.

The study is in a very early stage of an ongoing open-label, single-arm trial, at multiple sites in Europe, evaluating the anti-tumor effect of IXEMPRA in patients with locally recurrent or metastatic breast cancer after previous chemotherapies, including a taxane and an anthracycline. The included patients received a maximum of three prior lines of chemotherapies in the metastatic setting. Allarity recently amended the clinical trial protocol, lowering the DRP cut-off score in order to include more likely responder patients while still excluding those unlikely to respond to the drug. The ultimate objective is to further refine the DRP-IXEMPRA CDx criteria and broaden the enrollment of mBC patients who may substantially benefit from this treatment. The Company anticipates an additional interim data readout before the end of this year.

The DRP-IXEMPRA CDx is a transcriptomic signature comprising 191 mRNA biomarkers that are collectively predictive of tumor sensitivity or resistance to IXEMPRA. Using the DRP CDx to select likely responder patients while excluding likely resistant ones, Allarity aims to improve the benefit-risk ratio of IXEMPRA in metastatic or locally advanced breast cancer. The U.S. FDA-approved IXEMPRA label currently indicates a monotherapy efficacy with an objective response rate (ORR) of 12.4% and a clinical benefit rate (CBR) of 24.8% in metastatic or locally advanced breast cancer. However, the initial data from the ongoing DRP-guided Phase 2 study of IXEMPRA suggest that the DRP-IXEMPRA CDx may identify a subset of patients who potentially have an improved ORR and CBR as compared to monotherapy efficacy indicated by the U.S. FDA-approved label for the drug. The DRP-IXEMPRA CDx is a clinical stage companion diagnostic candidate and has not yet been approved by the U.S. FDA or the EMEA. Early trial results are insufficient to show statistical significance and may not be a reliable indicator of subsequent trial results based on a larger patient population.

IXEMPRA was originally developed by Bristol Myers Squibb and is approved for metastatic breast cancer patients in the U.S., where it is marketed by R-PHARM U.S., LLC. Allarity has the exclusive option rights for the development and commercialization of IXEMPRA in Europe.

Allarity’s Chief Executive Officer, James G. Cullem, further stated, "We are encouraged by these promising, early clinical data suggesting that mBC patients selected for treatment with IXEMPRA using our DRP companion diagnostic candidate for the drug mayhave substantially improved clinical benefit versus unselected patients. Allarity looks forward to fully enrolling and completing our ongoing Phase 2 trial for IXEMPRA, and remains enthusiastic about advancing this program towards market approval in Europe and, if approved, providing European mBC patients with first-time access to this beneficial drug."

Aeterna Zentaris to Participate in the Virtual Investor Summer Spotlight Series

On July 5, 2023 Aeterna Zentaris Inc. (NASDAQ: AEZS) (TSX: AEZS) ("Aeterna" or the "Company"), a specialty biopharmaceutical company developing and commercializing a diversified portfolio of pharmaceutical and diagnostic products, reported that Dr. Klaus Paulini, Chief Executive Officer and Eckhard Guenther, PhD, Managing Director and SVP, Business Development of Aeterna Zentaris, will participate in the Virtual Investor Summer Spotlight Series on Wednesday, July 13, 2023, at 10:00 AM ET (Press release, AEterna Zentaris, JUL 5, 2023, View Source;id=269419&p=2275749&I=1206939-c7Z3G6f3m8 [SID1234633060]).

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A live video webcast of the presentation will be available on the Events page of the Investors section of the Company’s website (zentaris.com). A webcast replay will be available two hours following the live presentation and will be accessible for 90 days.

AIM ImmunoTech to Participate in the Virtual Investor Summer Spotlight Series

On July 5, 2023 AIM ImmunoTech Inc. (NYSE American: AIM) ("AIM" or the "Company"), an immuno-pharma company focused on the research and development of therapeutics to treat multiple types of cancers, immune disorders, and viral diseases, including COVID-19, the disease caused by the SARS-CoV-2 virus, reported that Thomas K. Equels, MS JD, Chief Executive Officer of AIM ImmunoTech and Dr. Christopher McAleer, Scientific Officer at AIM ImmunoTech will participate in the Virtual Investor Summer Spotlight Series on Wednesday, July 12, 2023 at 11:00 AM ET (Press release, AIM ImmunoTech, JUL 5, 2023, View Source [SID1234633059]).

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A live video webcast of the presentation will be available on the Events page of the Company’s website (aimimmuno.com). A webcast replay will be available two hours following the live presentation and will be accessible for 90 days.